The Hulke Blog

2016 Update – Our Interns and Sunflower are Maturing!

2016 Update – Our Interns and Sunflower are Maturing!

As we transitioning to harvest season, lets take a look back at our projects and accomplishments from the summer. We had field locations in: Courtland, MN; Glyndon, MN; Galchutt, ND; Mandan, ND; and Oakes, ND. The field locations made up about 25 acres that we completed our research on. Some of the highlights from our summer were: Testing out our new seed indexer for planting Laying out 9 miles of drip tape in 1 day Field tours and visiting scientist Seeing…

Read More Read More

Genome Canada Progress

Genome Canada Progress

One of our big projects this summer was funded by Genome Canada, and involved us having a dryland trial and a flood trial out near Oakes, ND. In order to set up the flood trial, we had to manually lay irrigation drip tape (totaling about 9 miles!) to simulate the flood environment.  Below is a brief summary of the research project: Plants are regularly challenged by a variety of environmental stresses such as drought, flooding, salt, and low-nutrient levels. These stresses…

Read More Read More

PhD Student Visit

PhD Student Visit

Last week, our PhD student Cloe Pogoda visited our lab up in Fargo, ND.  She is being co-advised by Nolan Kane at CU-Boulder and Brent Hulke at our lab.  Cloe is pursuing her PhD in the Molecular, Cellular, and Developmental Biology (MCDB) department at Colorado, and is interested in using genomic tools to address a variety of ecological and evolutionary questions in a range of biological systems including sunflower. Cloe will be helping us with a wide variety of projects,…

Read More Read More

RHA 476 Release

RHA 476 Release

Our lab is proud to release a new sunflower line, RHA 476.  It has the potential to be the best line available in western Canada.  Check out the release below, along with the performance data table at the bottom: USDA has been working with Agriculture and Agri-Food Canada on development and evaluation of sunflower lines and hybrids for the western Canadian prairies.  We have been successful in developing an inbred line (RHA 476) that has superior yield potential and good…

Read More Read More

Shout-out to our intern!

Shout-out to our intern!

As part of our lab’s research, we often utilize a gas chromatograph (GC), which is an analytical instrument that helps analyze the content of various components in a sample.  In our case, we use this instrument to measure the oil content of a given sunflower variety.  We typically run about 5,000 or more samples per year.  Here is a sample output from the GC for five varieties: In order to prepare the numerous samples for gas chromatography, the samples must…

Read More Read More

MySQL Database Solution

MySQL Database Solution

I have set up a dedicated mySQL database system for the Hulke Lab. It will allow us to much more easily access nursery, field phenotyping trial, and genomic data sets to conduct model fitting. MySQL (Structured Query Language) is the most widely used open­source relational database management system (RDBMS). The first version of mySQL appeared in 1995, and Oracle operates it since 2010. MySQL is a fast, reliable, safe database system, and it works on many different platforms, such as Linux, Windows, OS. I recently set up…

Read More Read More

14th Annual National Sclerotinia Initiative Meeting

14th Annual National Sclerotinia Initiative Meeting

Last week, Brent attended the 14th annual national Sclerotinia initiative meeting (Minneapolis, MN) and gave a talk on our lab’s related Sclerotinia project. The title of his talk was “Using genomic selection to optimize prediction of Sclerotinia and agronomic phenotypes for more efficient breeding”. The primary goal of the Sclerotinia project is to better balance the intensity and efficiency of selection for Sclerotinia resistance and other agronomic traits, and to make more breeding progress per generation on all traits relative to their actual value to the…

Read More Read More

Unified SNP Map of Sunflower

Unified SNP Map of Sunflower

Dense genetic maps are critical tools for plant breeders and geneticists. While many maps have been developed for sunflower in the last few decades, most have been based on lowthroughput technologies and include marker numbers in the hundreds. However, two maps with reasonably dense coverage of about 5000 and 9000 single nucleotide polymorphism (SNP) loci each have recently been produced using high-throughput genotyping methods. Unfortunately, no mapping population is common between the two maps, making the development of a joint…

Read More Read More

Release of High Oleic, Low Saturated Lines

Release of High Oleic, Low Saturated Lines

The Hulke Lab is proud to announce four recent oilseed sunflower germplasms, HOLS1, HOLS2, HOLS3, and HOLS4.  These oilseeds were developed to provide increased diversity in fatty acid composition in the seed’s oil, elevated oleic acid levels above current high oleic inbred lines, and reduced saturated fatty acid composition.  The recurrent parent of the genetic stocks being released have resistance to Sclerotinia head rot (Sclerotinia sclerotiorum), and also to imidazolinone herbicides.  The seed will be available this Fall in time…

Read More Read More

Our Trip to The Land Institute

Our Trip to The Land Institute

Last week, Brent and Brian took a trip down to Kansas to visit The Land Institute.  While in Salina, they met with David Van Tassel to discuss the future of the Silphium domestication project.   Our lab will be working with a few people across the country, including the Kane Lab at Colorado.   This project will be focused on two specific species in the Silphium genus native to North America, Silphium integrifolium (whole-leaf rosinweed) and Silphium perfoliatum (cup plant).  It is our hope that a domesticated…

Read More Read More